Simultaneous determination of 14 bioactive citrus flavonoids using thin-layer chromatography combined with surface enhanced Raman spectroscopy

Citrus flavonoids consist of diverse analogs and possess various health-promoting effects dramatically depending on their chemical structures. Since different usually co-exist in real samples, it’s necessary to develop rapid efficient methods for simultaneous determination multiple flavonoids. Thin layer chromatography combined with surface enhanced Raman spectroscopy (TLC-SERS) was established simultaneously separate detect 14 citrus the first time. These target compounds could be characterized discriminated when paired SERS at 6–500 times greater sensitivity than TLC alone. TLC-SERS exhibited high recovery rates (91.5–121.7%) relative standard deviation lower 20.8%. Moreover, method successfully used which comparable accuracy performance liquid shorter analytical time (10 vs 45 min). All results demonstrated that this a promising simultaneous, rapid, sensitive accurate detection